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KMID : 1134820170460030320
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 3 p.320 ~ p.326
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Inhibitory Effects on Melanin Production in B16 Melanoma Cells of Fallen Pear
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Shin Bo-Yeon
Jung Bo-Ram
Jung Jong-Gi
Cho Seung-Sik
Bang Mi-Ae
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Abstract
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This study investigated the water extracts of fallen pear (FPWE) on tyrosinase activity and melanogenesis. In the present study, we examined the effects of FPWE on mushroom tyrosinase activity in vitro, B16F10 melanoma cell tyrosinase activity, melanin contents, and expression of melanogenic enzyme proteins such as tyrosinase. An apparent down-regulatory effect on tyrosinase activity was observed when B16F10 cells were incubated with FPWE. Results of melanin assay using B16F10 cells treated with different concentrations (50, 125, and 250 μg/mL) of FPWE showed a dose-dependent decrease in melanin content. To determine whether or not FPWE indirectly affects tyrosinase activity, we assessed mushroom tyrosinase activity upon treatment with various concentrations (125, 250, 500, and 1,000 μg/mL) of FPWE. In addition, we investigated changes in the protein level of tyrosinase by using Western blotting. Tyrosinase and microphthalmia-associated transcription factor expression levels in B16F10 melanoma cells were reduced in a dose-dependent manner by FPWE. These results suggest that FPWE reduced melanin formation by inhibition of tyrosinase activity. Therefore, we suggest that FPWE could be used an effective whitening agent for skin.
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KEYWORD
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fallen pear, B16F10 melanoma cell, melanin, tyrosinase, mushroom tyrosinase
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